Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Pantothenate

Calcium-pantothenate is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium-pantothenate or to replace this vitamin.

Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate.

Suspend 1.9 gram powdered medium in 1 litre distilled water

Store dry at room temperature