FoxO1 (phospho Ser256) rabbit pAb

FoxO1 (phospho Ser256) rabbit pAb

Vendor: ELK Biotechnology

SKU: ES5300

$148.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Applications
WB;IHC;IF;ELISA
Cellular localization
Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation
Clonality
Polyclonal
Concentration
1 mg/ml
Geneid human
2308
Human swiss prot no
Q12778
Immunogen
The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272
Isotype
IgG
Observed band
82kD
Product weight
0.005kg
Reactivity
Human;Mouse;Rat;Drosophila
Recommended dilutions
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Source
Rabbit
Storage
-20°C/1 year
FoxO1 (phospho Ser256) rabbit pAb

FoxO1 (phospho Ser256) rabbit pAb

$148.00 USD