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Goat OC(Osteocalcin) ELISA Kit

Goat OC(Osteocalcin) ELISA Kit

Regular price $588.00 USD
Sale price Regular price $0.00 USD

THE SCIENCE, IN DETAIL

Know your product.

Specifications
Assay length
3.5h
Assay type
Sandwich
Detection range
0.78-50 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Goat
Research area
Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Sample type
serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.23 ng/mL
Standard
50 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat OC in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P0C225
Technical documents

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