High Density Nickel

Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow). REFERENCES Lechner, Horst et al. “Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations.” Biotechnology Journal 13.3 (2018): n. pag. Biotechnology Journal. Marsden, A. P. et al. Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome. Journal of Molecular Biology 430, 5207–5216 (2018). Sanchez-Rodriguez, Ana et al. “Recombinant Rabbit Beta Nerve Growth Factor Production and Its Biological Effects on Sperm and Ovulation in Rabbits.” PLoS ONE 14.7 (2019): n. pag. PLoS ONE.