This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

around 3 working days

L 16cm x W 15cm x H 9cm

Metabolic pathway;Developmental science;Bone metabolism;

2h

serum, plasma, cell lysates, cell culture supernates and other biological fluids

23.44-1500 ng/mL

1500 ng/mL

7.29 ng/mL

Competitive Inhibition

Horse