Human C3(Complement Component 3) ELISA Kit

Item number: ELK11059
Availability: In Stock
Product Type: Precoated
$265.00
$265.00
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Size: 48T
Subtotal: $265.00
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Human C3(Complement Component 3) ELISA Kit

Human C3(Complement Component 3) ELISA Kit

$265.00

Human C3(Complement Component 3) ELISA Kit

$265.00
Size: 48T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P01024
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Signal transduction;Infection immunity;Immune molecule;
Assay length
3.5h
Sample type
serum, plasma and other biological fluids
Detection range
0.32-20 µg/mL
Standard
20 µg/mL
Sensitivity
0.11 µg/mL
Assay type
Sandwich
Reactivity
Human

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