Human CHI3L1(Chitinase-3-Like Protein 1) ELISA Kit
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$320.00 USD
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Regular price
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For research use only — not intended for clinical or diagnostic purposes.
Product Overview
Product Overview
Assay length
3.5h
Assay type
Sandwich
Detection range
0.78-50 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Metabolic pathway;
Sample type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.18 ng/mL
Standard
50 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHI3L1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CHI3L1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CHI3L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CHI3L1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
Background
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More Info
Human CHI3L1(Chitinase-3-Like Protein 1) ELISA Kit
Regular price
$320.00 USD
Sale price
Regular price
Unit price
/per

