Human CK 18-M30 (Cytokeratin 18-M30) ELISA Kit

Name: Human CK 18-M30 (Cytokeratin 18-M30) ELISA Kit
Brand: ELK Biotechnology
Price: 458.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK0871

$458.00 USD

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

15.63-1000 µIU/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Human

Research area

Cancer of the liver; Viral hepatitis

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

7 µIU/mL

Standard

1000 µIU/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M30 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human CK 18-M30 (Cytokeratin 18-M30) ELISA Kit

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