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Human MEGF8(Multiple Epidermal Growth Factor-Like Domains Protein 8) ELISA Kit

Human MEGF8(Multiple Epidermal Growth Factor-Like Domains Protein 8) ELISA Kit

Regular price $458.00 USD
Sale price Regular price $0.00 USD

THE SCIENCE, IN DETAIL

Know your product.

Specifications
Assay length
3.5h
Assay type
Sandwich
Detection range
0.16-10 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Signal transduction;
Sample type
serum, plasma, saliva, tears and other biological fluids and other biological fluids
Sensitivity
0.063 ng/mL
Standard
10 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEGF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEGF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEGF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEGF8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q7Z7M0
Technical documents

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