Human NELL1 (Protein kinase C-binding protein NELL1) ELISA Kit

Item number: ELK9986
Availability: In Stock
Product Type: Precoated
$588.00
$588.00
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Size: 96T
Subtotal: $588.00
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Human NELL1 (Protein kinase C-binding protein NELL1) ELISA Kit

Human NELL1 (Protein kinase C-binding protein NELL1) ELISA Kit

$588.00

Human NELL1 (Protein kinase C-binding protein NELL1) ELISA Kit

$588.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NELL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NELL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NELL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NELL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q92832
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Neuroscience
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates and other biological fluids
Detection range
31.25-2000 pg/mL
Standard
2000 pg/mL
Sensitivity
13.5 pg/mL
Assay type
Sandwich
Reactivity
Human

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