Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Name: Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Brand: ELK Biotechnology
Price: 376.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK1212

$376.00 USD

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

39.07-2500 pg/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Human

Research area

Endocrinology;Cardiovascular biology;

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

15 pg/mL

Standard

2500 pg/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

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