Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Item number: ELK1212
Availability: In Stock
Product Type: Precoated
$376.00
$376.00
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Size: 96T
Subtotal: $376.00
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Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

$376.00

Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

$376.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Endocrinology;Cardiovascular biology;
Assay length
3.5h
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection range
39.07-2500 pg/mL
Standard
2500 pg/mL
Sensitivity
15 pg/mL
Assay type
Sandwich
Reactivity
Human

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