Human SP-D(Pulmonary surfactant-associated protein D) ELISA Kit
Human SP-D(Pulmonary surfactant-associated protein D) ELISA Kit

Human SP-D(Pulmonary surfactant-associated protein D) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK8561

$265.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
1.25-80 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Infection immunity;Pulmonology;
Sample type
serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
Sensitivity
0.53 ng/mL
Standard
80 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SP-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SP-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P35247
Human SP-D(Pulmonary surfactant-associated protein D) ELISA Kit

Human SP-D(Pulmonary surfactant-associated protein D) ELISA Kit

$265.00 USD