Human TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
Human TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Human TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK9097

$320.00 USD
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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
2h
Assay type
Competitive Inhibition
Detection range
6.25-400 nmol/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Metabolic pathway;Hepatology;Hormone metabolism;
Sample type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
3.58 nmol/mL
Standard
400 nmol/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.
Human TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

Human TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

$320.00 USD