Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit - Vareum scientific
Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit - Vareum scientific
Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit - Vareum scientific
Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit - Vareum scientific

Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK1190

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$376.00 USD
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For research use only — not intended for clinical or diagnostic purposes.
Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit - Vareum scientific

Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit

$376.00 USD

THE SCIENCE, IN DETAIL

Know your product.

Specifications
Assay length
3.5h
Assay type
Sandwich
Detection range
15.63-1000 pg/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Human
Research area
Cytokine;Tumor immunity;Infection immunity;
Sample type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
6.4 pg/mL
Standard
1000 pg/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P01375
Technical documents

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