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HVA(Homovanillic Acid) ELISA Kit

HVA(Homovanillic Acid) ELISA Kit

Regular price $265.00 USD
Sale price Regular price

THE SCIENCE, IN DETAIL

Know your product.

Specifications
Assay length
2h
Assay type
Competitive Inhibition
Detection range
3.13-200 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
General
Research area
Neuroscience
Sample type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.96 ng/mL
Standard
200 ng/mL
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVA in the samples is then determined by comparing the OD of the samples to the standard curve.
Technical documents

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