Western Blot, Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, All lanes: ITCH antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 103, 99, 87 kDa, Observed band size: 103 kDa
Immunofluorescence staining of HepG2 cells with CAC15758 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ITCH Polyclonal Antibody - image 3
Western Blot, Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, All lanes: ITCH antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 103, 99, 87 kDa, Observed band size: 103 kDa
Immunofluorescence staining of HepG2 cells with CAC15758 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
ITCH Polyclonal Antibody - image 3

ITCH Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC15758-100UL

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q96J02
Applications
ELISA, WB, IF
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The ITCH Polyclonal Antibody is applicable for the following applications: ELISA, WB, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA)
Isotype
IgG
Purification
>95%, Protein G purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
ITCH
Target synonyms
ADMFD; AIF4; AIP4; Atrophin 1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); dJ468O1.1 atrophin 1 interacting protein 4 AIP4; E3 ubiquitin protein ligase Itchy homolog; E3 ubiquitin-protein ligase Itchy homolog; EC 6.3.2; Itch; ITCH_HUMAN; Itchy E3 ubiquitin protein ligase; Itchy E3 ubiquitin protein ligase homolog; Itchy E3 ubiquitin protein ligase homolog mouse; Itchy E3 ubiquitin protein ligase; mouse; homolog of; Itchy homolog E3 ubiquitin protein ligase; Itchy mouse homolog E3 ubiquitin protein ligase; NAPP1; NFE2 associated polypeptide 1; NFE2-associated polypeptide 1; Ubiquitin protein ligase ITCH
Western Blot, Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, All lanes: ITCH antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 103, 99, 87 kDa, Observed band size: 103 kDa

ITCH Polyclonal Antibody

$510.25 USD