IHC image of CAC15779 diluted at 1:300 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC15779 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: COLO320 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, All lanes: KRT20 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 49 kDa, Observed band size: 49 kDa
KRT20 Polyclonal Antibody - image 4
IHC image of CAC15779 diluted at 1:300 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC15779 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot, Positive WB detected in: COLO320 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, All lanes: KRT20 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 49 kDa, Observed band size: 49 kDa
KRT20 Polyclonal Antibody - image 4

KRT20 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC15779-100UL

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P35900
Applications
ELISA, WB, IHC, IF
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The KRT20 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
Recombinant Human Keratin, type I cytoskeletal 20 protein (207-424AA)
Isotype
IgG
Purification
>95%, Protein G purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
KRT20
Target synonyms
CD20; CK 20; CK-20; CK20; Cytokeratin-20; Cytokeratin20; K1C20_HUMAN; K20; KA20; Keratin 20; keratin 20; type I; keratin 21; rat; homolog of; Keratin; Keratin type I cytoskeletal 20; Keratin-20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; OTTHUMP00000164518; Protein IT; type I cytoskeletal 20
IHC image of CAC15779 diluted at 1:300 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

KRT20 Polyclonal Antibody

$510.25 USD