LbaCas12a Nuclease

Name: LbaCas12a Nuclease
Brand: Novoprotein
Price: 214.0 USD
Availability: InStock

Vendor: Novoprotein

Type: Molecular

SKU: E373-YH01

$214.00 USD

Shipping calculated at checkout.

Documents

Datasheet

Available in stock (500)

Customs clearance fees may apply
Worldwide shipping available.

For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Applications

Molecular Diagnostics

Description

Ribonucleoprotein (RNP)-mediated CRISPR genome editing is more effective and avoids the problems associated with using plasmids. Significantly reduce off-target effects.

Shipping conditions

Ice Pack

Storage

-20℃

Background

Cas12a (previously known as Cpf1) belongs to the Class 2 Type V CRISPR/Cas system. Different from Cas9 protein, Cas12a does not require tracrRNA, but only a single crRNA guide consisting of 41-44nt nucleotides. In contrast to Cas9, which requires a G-rich PAM, the Cas12a-crRNA complex recognizes a T-rich (5'-TTTN-3') protospacer adjacent motif (PAM) and results in a staggered DNA double-strand break (DSB). After the specific cleavage, Cas12a can also activate collateral cleavage activity towards adjacent non-specific ssDNA sequences. Hence, Cas12a nuclease is a good alternative for Cas9 in certain target DNA editing, and provides a novel strategy for DNA detection. LbaCas12a Nuclease is an RNA-guided DNA endonuclease from Lachnospiraceae bacterium, with a nuclear localization signal (NLS) added to both ends of the protein.

LbaCas12a Nuclease

Documents

Product Overview

Background

Documents

Documents

Product Citations

More Info