Western Blot, Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, HL60 whole cell lysate, All lanes: MDM2 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 56, 34, 31, 25, 36, 15, 12, 49, 50 kDa, Observed band size: 56 kDa
IHC image of CAC15788 diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
MDM2 Polyclonal Antibody - image 3
Western Blot, Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, HL60 whole cell lysate, All lanes: MDM2 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 56, 34, 31, 25, 36, 15, 12, 49, 50 kDa, Observed band size: 56 kDa
IHC image of CAC15788 diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
MDM2 Polyclonal Antibody - image 3

MDM2 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC15788-100UL

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q00987
Applications
ELISA, WB, IHC
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The MDM2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Peptide sequence from Human E3 ubiquitin-protein ligase Mdm2 protein (374-391AA)
Isotype
IgG
Purification
>95%, Protein G purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
MDM2
Target synonyms
ACTFS; Double minute 2 protein; E3 ubiquitin-protein ligase Mdm2; Hdm 2; Hdm2; HDMX; MDM 2; MDM2; MDM2 oncogene E3 ubiquitin protein ligase; Mdm2 p53 E3 ubiquitin protein ligase homolog; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa; MDM2_HUMAN; MDM2BP; Mouse Double Minute 2; MTBP; Murine Double Minute Chromosome 2; Oncoprotein Mdm2; p53 Binding Protein Mdm2; p53-binding protein Mdm2; Ubiquitin protein ligase E3 Mdm2; Ubiquitin protein ligase E3 Mdm2
Western Blot, Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, HL60 whole cell lysate, All lanes: MDM2 antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 56, 34, 31, 25, 36, 15, 12, 49, 50 kDa, Observed band size: 56 kDa

MDM2 Polyclonal Antibody

$510.25 USD