Mouse Anti-KLH IgG(Keyhole Limpet Hemocyanin IgG) ELISA Kit

Name: Mouse Anti-KLH IgG(Keyhole Limpet Hemocyanin IgG) ELISA Kit
Brand: ELK Biotechnology
Price: 458.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK0111

$458.00 USD

~~$0.00 USD~~

Shipping calculated at checkout.

Documents

Manuals

SDS

Supplier image

Available in stock (100)

Customs clearance fees may apply
Worldwide shipping available.

For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

1.57-100 ng/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Mouse

Research area

Immunology

Sample type

Serum, plasma, tissue homogenates

Sensitivity

0.67 ng/mL

Standard

100 ng/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-KLH IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-KLH IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-KLH IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-KLH IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse Anti-KLH IgG(Keyhole Limpet Hemocyanin IgG) ELISA Kit

Documents

Product Overview

Background

Documents

Documents

Product Citations

More Info