Mouse CD80(T-lymphocyte Activation Antigen CD80) ELISA Kit

Name: Mouse CD80(T-lymphocyte Activation Antigen CD80) ELISA Kit
Brand: ELK Biotechnology
Price: 458.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK0505

$458.00 USD

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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

15.63-1000 pg/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Mouse

Research area

CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity;

Sample type

Serum, plasma, tissue homogenates and other biological fluids

Sensitivity

6.05 pg/mL

Standard

1000 pg/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD80. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD80. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD80, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD80 in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse CD80(T-lymphocyte Activation Antigen CD80) ELISA Kit

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