Mouse GAD2(Glutamate Decarboxylase 2, Acid) ELISA Kit

Name: Mouse GAD2(Glutamate Decarboxylase 2, Acid) ELISA Kit
Brand: ELK Biotechnology
Price: 458.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK0588

$458.00 USD

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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

78.13-5000 pg/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Mouse

Research area

Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;

Sample type

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

32.3 pg/mL

Standard

5000 pg/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAD2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse GAD2(Glutamate Decarboxylase 2, Acid) ELISA Kit

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