Mouse SREBP2(Sterol Regulatory Element-binding Protein 2) ELISA Kit

Item number: ELK0001
Availability: In Stock
Product Type: Precoated
$458.00
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Size: 96T
Subtotal: $458.00
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Mouse SREBP2(Sterol Regulatory Element-binding Protein 2) ELISA Kit

Mouse SREBP2(Sterol Regulatory Element-binding Protein 2) ELISA Kit

$0.00 $458.00

Mouse SREBP2(Sterol Regulatory Element-binding Protein 2) ELISA Kit

$0.00 $458.00
Size: 96T
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SREBP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SREBP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SREBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SREBP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
Q3U1N2
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Research area
Cardiovascular; Epigenetics and Nuclear Signaling; Cancer; Metabolism
Assay length
3.5h
Sample type
Tissue homogenates, cell lysates and other biological fluids
Detection range
0.32-20 ng/mL
Standard
20 ng/mL
Sensitivity
0.13 ng/mL
Assay type
Sandwich
Reactivity
Mouse

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