Western Blot; Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse spleen tissue; All lanes: NSUN2 antibody at 6.7µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 87, 83, 60 kDa; Observed band size: 87 kDa;
Immunofluorescence staining of Hela cells with CAC11327 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Nsun2 Polyclonal Antibody - image 4
Western Blot; Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse spleen tissue; All lanes: NSUN2 antibody at 6.7µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 87, 83, 60 kDa; Observed band size: 87 kDa;
Immunofluorescence staining of Hela cells with CAC11327 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Nsun2 Polyclonal Antibody - image 4

Nsun2 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC11327-100UG

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q08J23
Applications
ELISA, WB, IHC, IF
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Nsun2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Trna (Cytosine (34) -C (5)) -Methyltransferase Protein (432-509Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Rat (Rattus norvegicus), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
NSUN2
Target synonyms
5 methycytoisine methyltransferase; D13Wsu123e; FLJ20303; hTrm4; MISU; Myc induced SUN domain containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family 2 protein; NOL1/NOP2/Sun domain family member 2; NSUN 2; NSUN2; NSUN2_HUMAN; SAKI; Substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine 5) methyltransferase; tRNA (cytosine 5) methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolo; tRNA methyltransferase 4 homolog
Western Blot; Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse spleen tissue; All lanes: NSUN2 antibody at 6.7µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 87, 83, 60 kDa; Observed band size: 87 kDa;

Nsun2 Polyclonal Antibody

$510.25 USD