Western Blot, Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, HUVEC whole cell lysate, All lanes: NT5E antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 64, 58 kDa, Observed band size: 64 kDa
IHC image of CAC15737 diluted at 1:30 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
NT5E Polyclonal Antibody - image 3
Western Blot, Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, HUVEC whole cell lysate, All lanes: NT5E antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 64, 58 kDa, Observed band size: 64 kDa
IHC image of CAC15737 diluted at 1:30 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
NT5E Polyclonal Antibody - image 3

NT5E Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC15737-100UL

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P21589
Applications
ELISA, WB, IHC
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The NT5E Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human 5\'-nucleotidase protein (32-264AA)
Isotype
IgG
Purification
Antigen affinity purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cardiovascular
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
NT5E
Target synonyms
5' NT; 5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase; ecto; 5' nucleotidase; ecto (CD73); 5'-NT; 5'-nucleotidase; 5NTD_HUMAN; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5 Prime Nucleotidase
Western Blot, Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, HUVEC whole cell lysate, All lanes: NT5E antibody at 1:2000, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 64, 58 kDa, Observed band size: 64 kDa

NT5E Polyclonal Antibody

$510.25 USD