Western Blot; Positive WB detected in: K562 whole cell lysate; All lanes: PADI2 antibody at 3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 76, 50 kDa; Observed band size: 76, 50 kDa
IHC image diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC09963 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Padi2 Polyclonal Antibody - image 4
Western Blot; Positive WB detected in: K562 whole cell lysate; All lanes: PADI2 antibody at 3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 76, 50 kDa; Observed band size: 76, 50 kDa
IHC image diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC09963 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Padi2 Polyclonal Antibody - image 4

Padi2 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC09963-100UG

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q9Y2J8
Applications
ELISA, WB, IHC, IF
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Padi2 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Protein-Arginine Deiminase Type-2 Protein (1-665Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Neuroscience
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
PADI2
Target synonyms
KIAA0994; OTTHUMP00000044625; PAD 2; PAD H19; PAD-H19; PAD2; PADI 2; Padi2; PADI2 protein; PADI2_HUMAN; PDI 2; PDI2; Peptidlyarginine deiminase type II; Peptidyl arginine deiminase II; Peptidyl arginine deiminase type II; Peptidylarginine deiminase II; Protein arginine deiminase; Protein arginine deiminase type 2; Protein arginine deiminase type II; Protein-arginine deiminase type II; Protein-arginine deiminase type-2
Western Blot; Positive WB detected in: K562 whole cell lysate; All lanes: PADI2 antibody at 3µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 76, 50 kDa; Observed band size: 76, 50 kDa

Padi2 Polyclonal Antibody

$510.25 USD