Immunohistochemistry of paraffin-embedded human gastric cancer using CAC10817 at dilution of 1:100
IHC image diluted at 1:325 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC10817 at 1:108, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Pard6B Polyclonal Antibody - image 4
Immunohistochemistry of paraffin-embedded human gastric cancer using CAC10817 at dilution of 1:100
IHC image diluted at 1:325 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC10817 at 1:108, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Pard6B Polyclonal Antibody - image 4

Pard6B Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC10817-100UL

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q9BYG5
Applications
ELISA, IHC, IF
Buffer
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Pard6B Polyclonal Antibody is applicable for the following applications: ELISA, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Partitioning Defective 6 Homolog Beta Protein (253-372Aa)
Isotype
IgG
Purification
Antigen Affinity Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
PARD6B
Target synonyms
PARD6B; PAR6BPartitioning defective 6 homolog beta; PAR-6 beta; PAR-6B
Immunohistochemistry of paraffin-embedded human gastric cancer using CAC10817 at dilution of 1:100

Pard6B Polyclonal Antibody

$510.25 USD