Pig GDNF(Glial Cell Line Derived Neurotrophic Factor) ELISA Kit

Name: Pig GDNF(Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
Brand: ELK Biotechnology
Price: 320.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK10092

$320.00 USD

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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

0.16-10 ng/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Pig

Research area

Cytokine;Apoptosis;Neuro science;

Sample type

serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids

Sensitivity

0.058 ng/mL

Standard

10 ng/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig GDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig GDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig GDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig GDNF in the samples is then determined by comparing the OD of the samples to the standard curve.

Pig GDNF(Glial Cell Line Derived Neurotrophic Factor) ELISA Kit

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