Pig SPA(Surfactant Associated Protein A) ELISA Kit
Pig SPA(Surfactant Associated Protein A) ELISA Kit

Pig SPA(Surfactant Associated Protein A) ELISA Kit

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK5699

$412.00 USD
Shipping calculated at checkout.

Available in stock (100)

View UniProt Entry
  • Customs clearance fees may apply
  • Worldwide shipping available.
For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length
3.5h
Assay type
Sandwich
Detection range
3.13-200 ng/mL
Lead time for package collection
around 3 working days
Package dimensions
L 16cm x W 15cm x H 9cm
Reactivity
Pig
Research area
Infection immunity;Pulmonology;
Sample type
serum, plasma, lung lavage fluid, tissue homogenates and other biological fluids
Sensitivity
1.35 ng/mL
Standard
200 ng/mL
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig SPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig SPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig SPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig SPA in the samples is then determined by comparing the OD of the samples to the standard curve.
Uniprot id
P49874
Pig SPA(Surfactant Associated Protein A) ELISA Kit

Pig SPA(Surfactant Associated Protein A) ELISA Kit

$412.00 USD