Western Blot; Positive WB detected in: 293T whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue; All lanes: PIN4 antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 14, 17 kDa; Observed band size: 14 kDa
IHC image diluted at 1:800 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC11415 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Pin4 Polyclonal Antibody - image 4
Western Blot; Positive WB detected in: 293T whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue; All lanes: PIN4 antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 14, 17 kDa; Observed band size: 14 kDa
IHC image diluted at 1:800 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CAC11415 at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Pin4 Polyclonal Antibody - image 4

Pin4 Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC11415-100UG

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q9Y237
Applications
ELISA, WB, IHC, IF
Buffer
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Pin4 Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Peptidyl-Prolyl Cis-Trans Isomerase Nima-Interacting 4 Protein (1-131Aa)
Isotype
IgG
Purification
>95%, Protein G Purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
PIN4
Target synonyms
EPVH; Eukaryotic parvulin homolog; hEPVH; hPar14; hPar17; MGC138486; OTTHUMP00000023527; OTTHUMP00000217483; OTTHUMP00000217485; Par14; Par17; Parvulin 14; Parvulin-14; Parvulin-17; Peptidyl prolyl cis trans isomerase NIMA interacting 4; Peptidyl prolyl cis/trans isomerase EPVH; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4; Peptidyl-prolyl cis-trans isomerase PIN4; Peptidyl-prolyl cis/trans isomerase EPVH; PIN4; PIN4_HUMAN; PPIase Pin4; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting; 4 (parvulin); Rotamase Pin4
Western Blot; Positive WB detected in: 293T whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue; All lanes: PIN4 antibody at 5µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 14, 17 kDa; Observed band size: 14 kDa

Pin4 Polyclonal Antibody

$510.25 USD