Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, LO2 whole cell lysate, Mouse brain tissue, All lanes: PKM antibody at 4.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 58, 59, 57 kDa, Observed band size: 58 kDa
IHC image of CAC13867 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC13867 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC13867 at 1:175, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
PKM Polyclonal Antibody - image 5
Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, LO2 whole cell lysate, Mouse brain tissue, All lanes: PKM antibody at 4.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 58, 59, 57 kDa, Observed band size: 58 kDa
IHC image of CAC13867 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CAC13867 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with CAC13867 at 1:175, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
PKM Polyclonal Antibody - image 5

PKM Polyclonal Antibody

Vendor: Biomatik

Type: Antibodies

SKU: CAC13867-100UG

$510.25 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P14618
Applications
ELISA, WB, IHC, IF
Buffer
0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4
Clonality
Polyclonal
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The PKM Polyclonal Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
Recombinant Human Pyruvate kinase PKM protein (185-461AA)
Isotype
IgG
Purification
>95%, Protein G purified
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
PKM
Target synonyms
CTHBP; Cytosolic thyroid hormone-binding protein; KPYM_HUMAN; OIP-3; Opa-interacting protein 3; p58; pkm; PKM1; PKM2; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; THBP1; Thyroid hormone-binding protein 1; Tumor M2-PK
Western Blot, Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, LO2 whole cell lysate, Mouse brain tissue, All lanes: PKM antibody at 4.5ug/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 58, 59, 57 kDa, Observed band size: 58 kDa

PKM Polyclonal Antibody

$510.25 USD