pOET3 Transfer Plasmid

Product code: 200104 Company undertaking Identification: Oxford Expression Technologies Ltd, Unit 9 Innovation Quarter, Oxford Technology Park, Kidlington OX5 1GN, UK pOET3 (10ug) is a baculovirus transfer vector designed for high level expression of foreign genes under the late AcMNPV basic (p6.9) promoter. Using this promoter will provide earlier expression compared to the polyhedrin promoter. This has been shown to be beneficial when expressing proteins which require extensive post ­translational modifications i.e. glycosylation. The vector is smaller than other available transfer vectors (4530bp) which greatly facilitates the cloning steps. It has a ColE1 origin of replication and an ampicillin resistance gene for selection in E. coli. The polyhedrin sequences have been replaced by a multiple cloning site (MCS) containing unique restriction sites for insertion of the foreign gene in the correct orientation. The AcMNPV sequences flanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET3 is compatible with any baculovirus system that utilises homologous recombination in insect cells.