Rabbit CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

Name: Rabbit CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit
Brand: ELK Biotechnology
Price: 320.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK10099

$320.00 USD

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Available in stock (100)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

1.57-100 ng/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Rabbit

Research area

Enzyme & Kinase;Cardiovascular biology;

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

0.59 ng/mL

Standard

100 ng/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit CKMB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CKMB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit CKMB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CKMB in the samples is then determined by comparing the OD of the samples to the standard curve.

Rabbit CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

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