Rabbit IL1b(Interleukin 1 Beta) ELISA Kit

Name: Rabbit IL1b(Interleukin 1 Beta) ELISA Kit
Brand: ELK Biotechnology
Price: 528.0 USD
Availability: InStock

Vendor: ELK Biotechnology

Type: Precoated

SKU: ELK1228

$528.00 USD

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Assay length

3.5h

Assay type

Sandwich

Detection range

7.82-500 pg/mL

Lead time for package collection

around 3 working days

Package dimensions

L 16cm x W 15cm x H 9cm

Reactivity

Rabbit

Research area

Cytokine;Tumor immunity;Infection immunity;

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity

3 pg/mL

Standard

500 pg/mL

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Rabbit IL1b(Interleukin 1 Beta) ELISA Kit

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