Phospho-Cdk2 (Y15) Recombinant Antibody
Western Blot; Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not); All lanes:Phospho-CDK2 antibody at 0.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34 KDa; Observed band size: 34 KDa;
Immunocytochemistry analysis diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07011(3µg)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20µg);
Phospho-Cdk2 (Y15) Recombinant Antibody
Western Blot; Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not); All lanes:Phospho-CDK2 antibody at 0.8µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34 KDa; Observed band size: 34 KDa;
Immunocytochemistry analysis diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with Pervanadate; Lane 1: Rabbit control IgG(1µg)instead in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07011(3µg)+ Hela whole cell lysate treated with Pervanadate(1mg); Lane 3: Hela whole cell lysate treated with Pervanadate(20µg);

Phospho-Cdk2 (Y15) Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07011-100UL

$575.00 USD
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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P24941
Applications
ELISA, WB, IHC, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
2C4
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Phospho-Cdk2 (Y15) Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
A Synthesized Peptide Derived From Human Phospho-Cdk2 (Y15)
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
CDK2
Target synonyms
Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; CDK2; CDKN2
Phospho-Cdk2 (Y15) Recombinant Antibody

Phospho-Cdk2 (Y15) Recombinant Antibody

$575.00 USD