Atf4 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate; All lanes: ATF4 antibody at 1.6µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 39 KDa; Observed band size: 50 KDa;
IHC image diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with RAC07020 at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ATF4 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07020 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg);
Atf4 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate; All lanes: ATF4 antibody at 1.6µg/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 39 KDa; Observed band size: 50 KDa;
IHC image diluted at 1:160 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with RAC07020 at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating ATF4 in Hela whole cell lysate; , Lane 1: Rabbit control IgG instead in Hela whole cell lysate., For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); , Lane 2: RAC07020 (3µg) + Hela whole cell lysate (500µg); , Lane 3: Hela whole cell lysate (20µg);

Atf4 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07020-100UL

$575.00 USD
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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P18848
Applications
ELISA, WB, IHC, IF, FC, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
9.00E+01
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Atf4 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, FC, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Atf4
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
ATF4
Target synonyms
Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; ATF4; CREB2; TXREB
Atf4 Recombinant Antibody

Atf4 Recombinant Antibody

$575.00 USD