Mono-Methyl-Histone H3.1 (K18) Recombinant Antibody
Western Blot; Positive WB detected in MCF-7 whole cell lysate ela whole cell lysate epG2 whole cell lysate H-SY5Y whole cell lysate,Rat brain tissue; All lanes Mono-methyl-Histone H3.1(K18)antibody at 0.7ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 15 KDa; Observed band size: 15 KDa
Immunocytochemistry analysis of RAC07091 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07091 at 1:43,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Mono-Methyl-Histone H3.1 (K18) Recombinant Antibody
Western Blot; Positive WB detected in MCF-7 whole cell lysate ela whole cell lysate epG2 whole cell lysate H-SY5Y whole cell lysate,Rat brain tissue; All lanes Mono-methyl-Histone H3.1(K18)antibody at 0.7ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 15 KDa; Observed band size: 15 KDa
Immunocytochemistry analysis of RAC07091 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07091 at 1:43,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Mono-Methyl-Histone H3.1 (K18) Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07091-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P68431
Applications
ELISA, WB, ICC, IF
Buffer
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Clonality
Recombinant Monoclonal
Clone number
2B5
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Mono-Methyl-Histone H3.1 (K18) Recombinant Antibody is applicable for the following applications: ELISA, WB, ICC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
A Synthesized Peptide
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HIST1H3A
Target synonyms
Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ
Mono-Methyl-Histone H3.1 (K18) Recombinant Antibody

Mono-Methyl-Histone H3.1 (K18) Recombinant Antibody

$575.00 USD