Acetyl-Histone H2B Type 1-B (K20) Recombinant Antibody
Western Blot; Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min; All lanes Acetyl-Histone H2B type 1-B(K20)antibody at 0.135ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 15 KDa; Observed band size: 15 KDa
Immunocytochemistry analysis of RAC07103 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with RAC07103 at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Acetyl-Histone H2B Type 1-B (K20) Recombinant Antibody
Western Blot; Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min; All lanes Acetyl-Histone H2B type 1-B(K20)antibody at 0.135ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 15 KDa; Observed band size: 15 KDa
Immunocytochemistry analysis of RAC07103 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with RAC07103 at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Acetyl-Histone H2B Type 1-B (K20) Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07103-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P33778
Applications
ELISA, WB, ICC, IF, FC
Buffer
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Clonality
Recombinant Monoclonal
Clone number
21F11
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Acetyl-Histone H2B Type 1-B (K20) Recombinant Antibody is applicable for the following applications: ELISA, WB, ICC, IF, FC.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
A Synthesized Peptide
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HIST1H2BB
Target synonyms
Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BB; H2BFF
Acetyl-Histone H2B Type 1-B (K20) Recombinant Antibody

Acetyl-Histone H2B Type 1-B (K20) Recombinant Antibody

$575.00 USD