Arnt Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate; All lanes: ARNT antibody at 1.83ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 87, 85, 86 KDa; Observed band size: 87 KDa
IHC image of RAC07156 diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIF-1 beta in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07156 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07156 (3ug) + Hela whole cell lysate (500ug); Lane 3: Hela whole cell lysate (20ug)
Arnt Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate; All lanes: ARNT antibody at 1.83ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 87, 85, 86 KDa; Observed band size: 87 KDa
IHC image of RAC07156 diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunoprecipitating HIF-1 beta in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07156 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07156 (3ug) + Hela whole cell lysate (500ug); Lane 3: Hela whole cell lysate (20ug)

Arnt Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07156-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P27540
Applications
ELISA, WB, IHC, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
2F11
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Arnt Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Arnt
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cardiovascular
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
ARNT
Target synonyms
Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta; ARNT; BHLHE2
Arnt Recombinant Antibody

Arnt Recombinant Antibody

$575.00 USD