Aurkb Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; All lanes: AURKB antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 40, 36, 17, 35 kDa; Observed band size: 40 kDa
IHC image of RAC07169 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07169 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating AURKB in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07169 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07169(2ug)+ Hela whole cell lysate(500ug); Lane 3: Hela whole cell lysate (10ug)
Aurkb Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; All lanes: AURKB antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 40, 36, 17, 35 kDa; Observed band size: 40 kDa
IHC image of RAC07169 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07169 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating AURKB in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07169 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07169(2ug)+ Hela whole cell lysate(500ug); Lane 3: Hela whole cell lysate (10ug)

Aurkb Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07169-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
Q96GD4
Applications
ELISA, WB, IHC, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
4.00E+05
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Aurkb Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Aurora B
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling, Cancer, Cell Biology, Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
AURKB
Target synonyms
Aurora kinase B (EC 2.7.11.1) (Aurora 1) (Aurora- and IPL1-like midbody-associated protein 1) (AIM-1) (Aurora/IPL1-related kinase 2) (ARK-2) (Aurora-related kinase 2) (STK-1) (Serine/threonine-protein kinase 12) (Serine/threonine-protein kinase 5) (Serine/threonine-protein kinase aurora-B); AURKB; AIK2 AIM1 AIRK2 ARK2 STK1 STK12 STK5
Aurkb Recombinant Antibody

Aurkb Recombinant Antibody

$575.00 USD