Bcap31 Recombinant Antibody
Western Blot; Positive WB detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate; All lanes: BCAP31 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 28, 35 kDa; Observed band size: 28-36 kDa
IHC image of RAC07176 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with RAC07176 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Bcap31 Recombinant Antibody
Western Blot; Positive WB detected in: HepG2 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate; All lanes: BCAP31 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 28, 35 kDa; Observed band size: 28-36 kDa
IHC image of RAC07176 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cell with RAC07176 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Bcap31 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07176-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P51572
Applications
ELISA, WB, IHC, IF, FC
Buffer
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Clonality
Recombinant Monoclonal
Clone number
12G12
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Bcap31 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, FC.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Bcap31
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
BCAP31
Target synonyms
B-cell receptor-associated protein 31 (BCR-associated protein 31) (Bap31) (6C6-AG tumor-associated antigen) (Protein CDM) (p28); BCAP31; BAP31 DXS1357E
Bcap31 Recombinant Antibody

Bcap31 Recombinant Antibody

$575.00 USD