Chek1 Recombinant Antibody
Western Blot; Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate; All lanes: Chk1 antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 55, 44, 51 kDa; Observed band size: 55 kDa
IHC image of RAC07247 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with RAC07247 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Chek1 Recombinant Antibody
Western Blot; Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate; All lanes: Chk1 antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 55, 44, 51 kDa; Observed band size: 55 kDa
IHC image of RAC07247 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with RAC07247 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Chek1 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07247-100UL

$575.00 USD
Shipping calculated at checkout.

Documents

SDS

Available in stock (500)

  • Customs clearance fees may apply
  • Worldwide shipping available.
For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
O14757
Applications
ELISA, WB, IHC, IF
Buffer
{"type":"root","children":[{"type":"paragraph","children":[{"type":"text","value":"Rabbit IgG in Phosphate Buffered Saline, pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol"}]}]}
Clonality
Recombinant Monoclonal
Clone number
2F2
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Chek1 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Chk1
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling, Cancer
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
CHEK1
Target synonyms
Serine/threonine-protein kinase Chk1 (EC 2.7.11.1) (CHK1 checkpoint homolog) (Cell cycle checkpoint kinase) (Checkpoint kinase-1); CHEK1; CHK1
Chek1 Recombinant Antibody

Chek1 Recombinant Antibody

$575.00 USD