Lgals3 Recombinant Antibody
Western Blot; Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Colo320 whole cell lysate, HT29 whole cell lysate; All lanes: Galectin 3 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 27 kDa; Observed band size: 31 kDa
IHC image of RAC07358 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF7 Cells with RAC07358 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating LGALS3 in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07358 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07358(2ug)+ Hela whole cell lysate(500ug); Lane 3: Hela whole cell lysate (10ug)
Lgals3 Recombinant Antibody
Western Blot; Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Colo320 whole cell lysate, HT29 whole cell lysate; All lanes: Galectin 3 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 27 kDa; Observed band size: 31 kDa
IHC image of RAC07358 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of MCF7 Cells with RAC07358 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating LGALS3 in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07358 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07358(2ug)+ Hela whole cell lysate(500ug); Lane 3: Hela whole cell lysate (10ug)

Lgals3 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07358-100UL

$575.00 USD
Shipping calculated at checkout.

Documents

SDS

Available in stock (500)

  • Customs clearance fees may apply
  • Worldwide shipping available.
For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P17931
Applications
ELISA, WB, IHC, IF, IP
Buffer
{"type":"root","children":[{"type":"paragraph","children":[{"type":"text","value":"Rabbit IgG in Phosphate Buffered Saline, pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol"}]}]}
Clonality
Recombinant Monoclonal
Clone number
4.00E+01
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Lgals3 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Galectin 3
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Neuroscience, Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
LGALS3
Target synonyms
Galectin-3 (Gal-3) (35 kDa lectin) (Carbohydrate-binding protein 35) (CBP 35) (Galactose-specific lectin 3) (Galactoside-binding protein) (GALBP) (IgE-binding protein) (L-31) (Laminin-binding protein) (Lectin L-29) (Mac-2 antigen); LGALS3; MAC2
Lgals3 Recombinant Antibody

Lgals3 Recombinant Antibody

$575.00 USD