Hnf4A Recombinant Antibody
Western Blot; Positive WB detected in: HL-60 whole cell lysate, Rat Liver tissue lysate,; All lanes: HNF-4-alpha antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 52 kDa; Observed band size: 52 kDa
IHC image of RAC07409 diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.
Immunofluorescence staining of MCF-7 with RAC07409 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Hnf4A Recombinant Antibody
Western Blot; Positive WB detected in: HL-60 whole cell lysate, Rat Liver tissue lysate,; All lanes: HNF-4-alpha antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 52 kDa; Observed band size: 52 kDa
IHC image of RAC07409 diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.75% DAB.
Immunofluorescence staining of MCF-7 with RAC07409 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 526-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Hnf4A Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07409-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P41235
Applications
ELISA, WB, IHC, IF, FC
Buffer
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Clonality
Recombinant Monoclonal
Clone number
10A7
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Hnf4A Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, FC.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Hnf4A
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling, Cancer, Cardiovascular, Developmental Biology, Metabolism, Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Rat (Rattus norvegicus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HNF4A
Target synonyms
Hepatocyte nuclear factor 4-alpha (HNF-4-alpha) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) (TCF-14) (Transcription factor HNF-4); HNF4A; HNF4 NR2A1 TCF14
Hnf4A Recombinant Antibody

Hnf4A Recombinant Antibody

$575.00 USD