Hnrnpc Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate; All lanes: hnRNP C1 + C2 antibody at 0.66ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34, 33, 26, 28 KDa; Observed band size: 42 KDa
IHC image of RAC07411 diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07411 at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating hnRNP C1 + C2 in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07411 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07411 (3ug) + Hela whole cell lysate (500ug); Lane 3: Hela whole cell lysate (20ug)
Hnrnpc Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate; All lanes: hnRNP C1 + C2 antibody at 0.66ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 34, 33, 26, 28 KDa; Observed band size: 42 KDa
IHC image of RAC07411 diluted at 1:66.35 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07411 at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating hnRNP C1 + C2 in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07411 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07411 (3ug) + Hela whole cell lysate (500ug); Lane 3: Hela whole cell lysate (20ug)

Hnrnpc Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07411-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P07910
Applications
ELISA, WB, IHC, IF, FC, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
9G1
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Hnrnpc Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, FC, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Hnrnpc
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HNRNPC
Target synonyms
Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; HNRNPC; HNRPC
Hnrnpc Recombinant Antibody

Hnrnpc Recombinant Antibody

$575.00 USD