Hspd1 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue; All lanes: HSPD1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 62, 18 kDa; Observed band size: 60 kDa
IHC image of RAC07417 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07417 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating HSPD1 in HepG2 whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07417 in HepG2 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07417(3ug)+ HepG2 whole cell lysate(500ug); Lane 3: HepG2 whole cell lysate (10ug)
Hspd1 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue; All lanes: HSPD1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 62, 18 kDa; Observed band size: 60 kDa
IHC image of RAC07417 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of RAC07417 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating HSPD1 in HepG2 whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07417 in HepG2 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07417(3ug)+ HepG2 whole cell lysate(500ug); Lane 3: HepG2 whole cell lysate (10ug)

Hspd1 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07417-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P10809
Applications
ELISA, WB, IHC, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
3D8
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Hspd1 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Hsp60
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Isotype/Loading Controls, Tags And Cell Markers, Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
HSPD1
Target synonyms
60 kDa heat shock protein; mitochondrial (EC 3.6.4.9) (60 kDa chaperonin) (Chaperonin 60) (CPN60) (Heat shock protein 60) (HSP-60) (Hsp60) (HuCHA60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein); HSPD1; HSP60
Hspd1 Recombinant Antibody

Hspd1 Recombinant Antibody

$575.00 USD