Itgb1 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, U251 whole cell lysate; All lanes: Integrin beta-1/CD29 antibody at 1.9ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 89, 88, 92 KDa; Observed band size: 130 KDa
IHC image of RAC07456 diluted at 1:185 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07456 at 1:61, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Itgb1 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, U251 whole cell lysate; All lanes: Integrin beta-1/CD29 antibody at 1.9ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 89, 88, 92 KDa; Observed band size: 130 KDa
IHC image of RAC07456 diluted at 1:185 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with RAC07456 at 1:61, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Itgb1 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07456-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P05556
Applications
ELISA, WB, IHC, IF, FC
Buffer
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Clonality
Recombinant Monoclonal
Clone number
32A10
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Itgb1 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, FC.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Itgb1
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Signal Transduction
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
ITGB1
Target synonyms
Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD29; ITGB1; FNRB; MDF2; MSK12
Itgb1 Recombinant Antibody

Itgb1 Recombinant Antibody

$575.00 USD