Nthl1 Recombinant Antibody
Western Blot; Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate; All lanes: NTHL1 antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 35, 34, 33 kDa; Observed band size: 35 kDa
IHC image of RAC07555 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of PC-3 cell with RAC07555 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 604-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Nthl1 Recombinant Antibody
Western Blot; Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate; All lanes: NTHL1 antibody at 1:1000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 35, 34, 33 kDa; Observed band size: 35 kDa
IHC image of RAC07555 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of PC-3 cell with RAC07555 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 604-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Nthl1 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07555-100UL

$575.00 USD
Shipping calculated at checkout.

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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P78549
Applications
ELISA, WB, IHC, IF, FC
Buffer
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Clonality
Recombinant Monoclonal
Clone number
22H10
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Nthl1 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, FC.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Nthl1
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Epigenetics And Nuclear Signaling
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
NTHL1
Target synonyms
Endonuclease III-like protein 1 (hNTH1) (EC 3.2.2.-) (EC 4.2.99.18) (Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase) (DNA glycosylase/AP lyase); NTHL1; NTH1 OCTS3
Nthl1 Recombinant Antibody

Nthl1 Recombinant Antibody

$575.00 USD