Phospho-Rps6Kb1 (T421+S424) Recombinant Antibody
Western Blot; Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not); All lanes Phospho-RPS6KB1 antibody at 0.93ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 70 KDa; Observed band size: 70 KDa
Immunofluorescence staining of Hela cells with RAC07650 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-RPS6KB1 in Hela whole cell lysate; Lane 1: Rabbit control IgG(1ug)instead of RAC07650 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07650(3ug)+ Hela whole cell lysate(1mg); Lane 3: Hela whole cell lysate (20ug)
Phospho-Rps6Kb1 (T421+S424) Recombinant Antibody
Western Blot; Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not); All lanes Phospho-RPS6KB1 antibody at 0.93ug/ml; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 70 KDa; Observed band size: 70 KDa
Immunofluorescence staining of Hela cells with RAC07650 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating Phospho-RPS6KB1 in Hela whole cell lysate; Lane 1: Rabbit control IgG(1ug)instead of RAC07650 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07650(3ug)+ Hela whole cell lysate(1mg); Lane 3: Hela whole cell lysate (20ug)

Phospho-Rps6Kb1 (T421+S424) Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07650-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P23443
Applications
ELISA, WB, IF, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
3B6
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Phospho-Rps6Kb1 (T421+S424) Recombinant Antibody is applicable for the following applications: ELISA, WB, IF, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Immunogen
A Synthesized Peptide Derived From Human Phospho-Rps6Kb1 (T421+S424)
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Cell Biology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
RPS6KB1
Target synonyms
Ribosomal protein S6 kinase beta-1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA; RPS6KB1; STK14A
Phospho-Rps6Kb1 (T421+S424) Recombinant Antibody

Phospho-Rps6Kb1 (T421+S424) Recombinant Antibody

$575.00 USD