Tpt1 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue; All lanes: TPT1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 20, 16 kDa; Observed band size: 25 kDa
IHC image of RAC07778 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of SY5Y Cells with RAC07778 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating TCTP in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07778 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07778(2ug)+ Hela whole cell lysate(500ug); Lane 3: Hela whole cell lysate (10ug)
Tpt1 Recombinant Antibody
Western Blot; Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, RAW264.7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse liver tissue; All lanes: TPT1 antibody at 1:2000; Secondary; Goat polyclonal to rabbit IgG at 1/50000 dilution; Predicted band size: 20, 16 kDa; Observed band size: 25 kDa
IHC image of RAC07778 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4? overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of SY5Y Cells with RAC07778 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunoprecipitating TCTP in Hela whole cell lysate; Lane 1: Rabbit control IgG instead of RAC07778 in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000); Lane 2: RAC07778(2ug)+ Hela whole cell lysate(500ug); Lane 3: Hela whole cell lysate (10ug)

Tpt1 Recombinant Antibody

Vendor: Biomatik

Type: Antibodies

SKU: RAC07778-100UL

$575.00 USD
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Available in stock (500)

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For research use only — not intended for clinical or diagnostic purposes.

Product Overview

Accession number
P13693
Applications
ELISA, WB, IHC, IF, IP
Buffer
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Clonality
Recombinant Monoclonal
Clone number
10A9
Concentration
Lot specific. Please inquire or refer to the datasheet included in the product pouch.
Conjugate
Unconjugated. Conjugated antibodies are available (Biotin, FITC and HRP), please inquire.
Description
The Tpt1 Recombinant Antibody is applicable for the following applications: ELISA, WB, IHC, IF, IP.
Expiration
12 months
Format
Liquid
Host
Rabbit
Ihc validation
IHC Validated
Immunogen
A Synthesized Peptide Derived From Human Tctp
Isotype
IgG
Lead time for package collection
7-11 Business Days
Purification
Affinity-Chromatography
Quality systems
This product is manufactured at ISO 9001 certified facilities.
Research area
Immunology
Restrictions
For Research Use Only. Not for use in diagnostic procedures.
Shipping conditions
Ice packs
Species
Human (Homo sapiens)
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus)
Storage
2°C to 8°C for frequent use, -20°C for 12 months. Avoid repeated freeze/thaw cycles.
Target name
TPT1
Target synonyms
Translationally-controlled tumor protein (TCTP) (Fortilin) (Histamine-releasing factor) (HRF) (p23); TPT1
Tpt1 Recombinant Antibody

Tpt1 Recombinant Antibody

$575.00 USD